|Application ||WB, IHC-P, E, IHC-Fr|
|Reactivity||Human, Rat, Monkey, Dog, Cat|
|Dilution||IHC-P (2.5 µg/ml)|
|Other Names||Tryptase alpha/beta-1, Tryptase-1, 184.108.40.206, Tryptase I, Tryptase alpha-1, TPSAB1, TPS1, TPS2, TPSB1|
|Target/Specificity||Recognizes human mast cell tryptase, both alpha and beta isoforms. Is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology.|
|Reconstitution & Storage||+4°C or -20°C, Avoid repeated freezing and thawing. Store undiluted.|
|Precautions||TPSAB1 / Mast Cell Tryptase Antibody (clone AA1) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||TPS1, TPS2, TPSB1|
|Function||Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315).|
|Cellular Location||Secreted. Note=Released from the secretory granules upon mast cell activation.|
|Tissue Location||Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
Miller J.S.,et al.J. Clin. Invest. 84:1188-1195(1989).
Schwartz L.B.,et al.Submitted (MAR-1990) to the EMBL/GenBank/DDBJ databases.
Vanderslice P.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:3811-3815(1990).
Pallaoro M.,et al.J. Biol. Chem. 274:3355-3362(1999).
Wang H.W.,et al.Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
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