|Application ||WB, IHC-P, IF|
|Reactivity||Human, Mouse, Rat|
|Dilution||IHC-P (5 µg/ml), WB (0.5-1 µg/ml),|
|Other Names||Neurosecretory protein VGF, Neuroendocrine regulatory peptide-1, NERP-1, Neuroendocrine regulatory peptide-2, NERP-2, Antimicrobial peptide VGF[554-577], VGF|
|Target/Specificity||The immunogen is after amino acid 577 of O15240, so the antibody should only cross-react with full-length VGF and none of the processed peptides.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||VGF Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.|
|Cellular Location||Secreted. Cytoplasmic vesicle, secretory vesicle. Note=Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus|
|Tissue Location||Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells|
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Provided below are standard protocols that you may find useful for product applications.
May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. Antimicrobial peptide VGF[554-577]: Has bactericidal activity against M. luteus, and antifungal activity against P. Pastoris.
Canu N.,et al.Genomics 45:443-446(1997).
Hillier L.W.,et al.Nature 424:157-164(2003).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Sasaki K.,et al.Mol. Cell. Proteomics 12:700-709(2013).
Toshinai K.,et al.Cell. Mol. Life Sci. 66:1939-1945(2009).
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