|Application ||WB, IHC-P, IF, IHC-Fr|
|Reactivity||Human, Mouse, Rat, Guinea Pig|
|Dilution||IHC-Fr (1-2 µg/ml), IHC-P (10 µg/ml), WB (2-4 µg/ml)|
|Other Names||Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19|
|Target/Specificity||Recognizes the rod domain of keratin 19 (40kD). Reacts with the MCF-7 cells, which are known to contain keratin 19. It shows no reaction with the cells lacking keratin 19 such as A431 and HeLa. Species cross-reactivity: Human. Weak reactivity with ra ...|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||KRT19 / CK19 / Cytokeratin 19 Antibody (aa312-335, clone A53-B/A2.26) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.|
|Tissue Location||Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.|
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Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Stasiak P.C.,et al.Nucleic Acids Res. 15:10058-10058(1987).
Bader B.L.,et al.Eur. J. Cell Biol. 47:300-319(1988).
Eckert R.L.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:1114-1118(1988).
Stasiak P.C.,et al.J. Invest. Dermatol. 92:707-716(1989).
Whittock N.V.,et al.Biochem. Biophys. Res. Commun. 267:462-465(2000).
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