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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TRIM25 Antibody (C-Terminus)   

TRIM25 Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • IF - TRIM25 Antibody (C-Terminus) ALS12401
    Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/ml.
  • IHC - TRIM25 Antibody (C-Terminus) ALS12401
    Anti-TRIM25 antibody IHC of human brain, cortex.
  • ICC - TRIM25 Antibody (C-Terminus) ALS12401
    Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 20 µg/ml.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC, E
Primary Accession Q14258
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 71kDa
Dilution ICC (20 µg/ml), IHC-P (10 µg/ml), WB (1-2 µg/ml)
Additional Information
Gene ID 7706
Other Names E3 ubiquitin/ISG15 ligase TRIM25, 6.3.2.19, 6.3.2.n3, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147, TRIM25, EFP, RNF147, ZNF147
Target/Specificity a 19 amino acid peptide from near the carboxy terminus of human TRIM25
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsTRIM25 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM25
Synonyms EFP, RNF147, ZNF147
Function Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).
Cellular Location Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies
Tissue Location Expressed in breast tumors (at protein level). Ubiquitous.
Research Areas
Citations (0)

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Background

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.

References

Inoue S.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:11117-11121(1993).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Shimada N.,et al.Mol. Cell. Endocrinol. 218:147-153(2004).
Rush J.,et al.Nat. Biotechnol. 23:94-101(2005).
Nakasato N.,et al.Biochem. Biophys. Res. Commun. 351:540-546(2006).

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$ 395.00
Cat# ALS12401
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