|Application ||IHC-P, IF, IHC-Fr|
|Dilution||IHC-Fr, IHC-P (20 µg/ml),|
|Other Names||Uromodulin, Tamm-Horsfall urinary glycoprotein, THP, Uromodulin, secreted form, UMOD|
|Target/Specificity||Anti-human Tamm-Horsfall protein (THP) is a monoclonal antibody which reacts with an epitope of the urinary mucoprotein. Tamm-Horsfall protein is a glycoprotein of approximately 80 kD containing up to 25% carbohydrate by weight.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||UMOD / Uromodulin Antibody (clone 10.32) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability (Probable). May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF (PubMed:3498215). Facilitates neutrophil migration across renal epithelia (PubMed:20798515).|
|Cellular Location||Apical cell membrane; Lipid- anchor, GPI-anchor. Basolateral cell membrane; Lipid-anchor, GPI-anchor. Cell projection, cilium membrane. Note=Only a small fraction sorts to the basolateral pole of tubular epithelial cells compared to apical localization (PubMed:22776760). Secreted into urine after cleavage (PubMed:18375198, PubMed:26811476). Colocalizes with NPHP1 and KIF3A (PubMed:20172860).|
|Tissue Location||Expressed in the tubular cells of the kidney. Most abundant protein in normal urine (at protein level) Synthesized exclusively in the kidney. Expressed exclusively by epithelial cells of the thick ascending limb of Henle's loop (TALH) and of distal convoluted tubule lumen|
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Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure providing the water barrier permeability. May serve as a receptor for binding and endocytosis for cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelial.
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Hart T.C.,et al.J. Med. Genet. 39:882-892(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Martin J.,et al.Nature 432:988-994(2004).
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