|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Sheep, Horse, Bovine, Guinea Pig|
|Dilution||IHC-P (5 µg/ml), WB (1 µg/ml),|
|Other Names||Cytochrome c, CYCS, CYC|
|Target/Specificity||Detects an ~14kD protein, corresponding to the apparent molecular mass of Cytochrome c on Western blots with samples from human, mouse, rat, bovine, guinea pig, hamster, monkey, rabbit and sheep origins. This antibody detects denatured Cytochrome c i ...|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||CYCS / Cytochrome c Antibody (clone 7H8.2C12) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.|
|Cellular Location||Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane|
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Provided below are standard protocols that you may find useful for product applications.
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Evans M.J.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:9625-9629(1988).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Hillier L.W.,et al.Nature 424:157-164(2003).
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