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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   BRAF / B-Raf Antibody (clone 1H12)   

BRAF / B-Raf Antibody (clone 1H12)

Mouse Monoclonal Antibody

     
  • IF - BRAF / B-Raf Antibody (clone 1H12) ALS13013
    Confocal immunofluorescence of MCF-7 (left) and HepG2 (right) cells using BRAF mouse monoclonal...
  • IHC - BRAF / B-Raf Antibody (clone 1H12) ALS13013
    Anti-BRAF antibody IHC of human brain, cerebellum.
  • IHC - BRAF / B-Raf Antibody (clone 1H12) ALS13013
    Anti-BRAF antibody IHC of human brain, cortex.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P15056
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 1H12
Calculated MW 84kDa
Dilution ELISA (1:10000), IF (1:200-1:1000), IHC-P (1:200-1:300), WB (1:500-1:2000) ,
Additional Information
Gene ID 673
Other Names Serine/threonine-protein kinase B-raf, 2.7.11.1, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
Target/Specificity Human BRAF
Reconstitution & Storage Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsBRAF / B-Raf Antibody (clone 1H12) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BRAF
Synonyms BRAF1, RAFB1
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Location Brain and testis.
Research Areas
Citations (0)

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Background

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

References

Stephens R.M.,et al.Mol. Cell. Biol. 12:3733-3742(1992).
Albert S.,et al.Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Scherer S.W.,et al.Science 300:767-772(2003).
Hillier L.W.,et al.Nature 424:157-164(2003).
Eychene A.,et al.Oncogene 7:1657-1660(1992).

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$ 395.00
Cat# ALS13013
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