|Application ||WB, IHC-P, E|
|Dilution||ELISA (1:10000), IHC-P (1:200-1:400), WB (1:500-1:2000)|
|Other Names||Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||AFP / Alpha Fetoprotein Antibody (clone 6E6) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.|
|Tissue Location||Plasma. Synthesized by the fetal liver and yolk sac|
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Provided below are standard protocols that you may find useful for product applications.
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Morinaga T.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:4604-4608(1983).
Gibbs P.E.M.,et al.Biochemistry 26:1332-1343(1987).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Sakai M.,et al.J. Biol. Chem. 260:5055-5060(1985).
McVey J.H.,et al.Hum. Mol. Genet. 2:379-384(1993).
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