|Application ||WB, IHC-P, E|
|Dilution||ELISA (1:32000), IHC-P (2.5 µg/ml), WB (0.1-0.3 µg/ml)|
|Other Names||Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1, SERPINE1, PAI1, PLANH1|
|Target/Specificity||Human SERPINE1 / PAI-1. This antibody is expected to recognise both reported isoforms.|
|Reconstitution & Storage||Store at -20°C. Minimize freezing and thawing.|
|Precautions||SERPINE1 / PAI-1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine protease inhibitor. Inhibits TMPRSS7 (PubMed:15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed:8481516, PubMed:9207454, PubMed:17912461). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed:9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed:15001579, PubMed:9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed:18386027). It is involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed:25808697, PubMed:27046084).|
|Tissue Location||Expressed in endothelial cells (PubMed:2430793, PubMed:3097076). Found in plasma, platelets, and hepatoma and fibrosarcoma cells.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
Pannekoek H.,et al.EMBO J. 5:2539-2544(1986).
Loskutoff D.J.,et al.Biochemistry 26:3763-3768(1987).
Ginsburg D.,et al.J. Clin. Invest. 78:1673-1680(1986).
Follo M.,et al.Gene 84:447-453(1989).
Strandberg L.,et al.Eur. J. Biochem. 176:609-616(1988).
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