|Application ||WB, IHC-P, E|
|Dilution||IHC-P (1:200), WB (1:500-1:2000),|
|Other Names||Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone, EGF|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||EGF Antibody (clone 9D7F11) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Expressed in kidney, salivary gland, cerebrum and prostate.|
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Provided below are standard protocols that you may find useful for product applications.
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
Bell G.I.,et al.Nucleic Acids Res. 14:8427-8446(1986).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Gregory H.,et al.Int. J. Pept. Protein Res. 9:107-118(1977).
Furuya M.,et al.Biochem. Biophys. Res. Commun. 163:1100-1106(1989).
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