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RCHY1 / PIRH2 Antibody (clone 1H10)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E, FC |
|---|---|
| Primary Accession | Q96PM5 |
| Reactivity | Human, Rat |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1H10 |
| Calculated MW | 30kDa |
| Dilution | ELISA (1:10000), Flo (1:200-1:400), IF (1:200-1:1000), IHC-P (1:200), WB (1:500-1:2000) |
| Gene ID | 25898 |
|---|---|
| Other Names | RING finger and CHY zinc finger domain-containing protein 1, 6.3.2.-, Androgen receptor N-terminal-interacting protein, CH-rich-interacting match with PLAG1, E3 ubiquitin-protein ligase Pirh2, RING finger protein 199, Zinc finger protein 363, p53-induced RING-H2 protein, hPirh2, RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 |
| Target/Specificity | Human RCHY1 |
| Reconstitution & Storage | Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles. |
| Precautions | RCHY1 / PIRH2 Antibody (clone 1H10) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RCHY1 |
|---|---|
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins, including p53/TP53, TP73, HDAC1 and CDKN1B (PubMed:19483087, PubMed:19043414, PubMed:16914734, PubMed:17721809, PubMed:18006823, PubMed:21994467). Mediates ubiquitination and degradation of p53/TP53; preferentially acts on tetrameric p53/TP53 (PubMed:19483087, PubMed:19043414). Catalyzes monoubiquitinates the translesion DNA polymerase POLH (PubMed:21791603). Involved in the ribosome-associated quality control (RQC) pathway, which mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes: RCHY1 acts downstream of NEMF and recognizes CAT tails associated with stalled nascent chains, leading to their ubiquitination and degradation (PubMed:33909987). |
| Cellular Location | Nucleus. Nucleus speckle. Cytoplasm |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
References
Corcoran C.A.,et al.J. Biol. Chem. 284:21955-21970(2009).
Beitel L.K.,et al.Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
Braem C.V.,et al.Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
Wu H.-S.,et al.Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
Wu G.,et al.FEBS Lett. 584:2772-2778(2010).
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