|Application ||IHC-P, E, IHC-Fr|
|Dilution||IHC-P (10 µg/ml)|
|Other Names||Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227, Mucin-1 subunit alpha, MUC1-NT, MUC1-alpha, Mucin-1 subunit beta, MUC1-beta, MUC1-CT, MUC1, PUM|
|Target/Specificity||Recognizes underglycosylated and natural MUC1 protein (isolated from human milk by affinity chromatography). Does not cross-react with normal serum proteins and human tissues of nonepithelial origin. Binds with high efficiency with different epitopes ...|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||EMA / MUC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.|
|Cellular Location||Apical cell membrane; Single- pass type I membrane protein. Note=Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions Isoform Y: Secreted. Mucin-1 subunit beta: Cell membrane. Cytoplasm. Nucleus. Note=On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus|
|Tissue Location||Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.|
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Provided below are standard protocols that you may find useful for product applications.
The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
Lan M.S.,et al.J. Biol. Chem. 265:15294-15299(1990).
Ligtenberg M.J.L.,et al.J. Biol. Chem. 265:5573-5578(1990).
Gendler S.J.,et al.J. Biol. Chem. 265:15286-15293(1990).
Lancaster C.A.,et al.Biochem. Biophys. Res. Commun. 173:1019-1029(1990).
Wreschner D.H.,et al.Eur. J. Biochem. 189:463-473(1990).
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