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SUPT16H / FACTP140 Antibody (Internal)

Goat Polyclonal Antibody

     
  • WB - SUPT16H / FACTP140 Antibody (Internal) ALS13428
    Antibody (0.1 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer).
  • IHC - SUPT16H / FACTP140 Antibody (Internal) ALS13428
    Anti-SUPT16H antibody IHC of human kidney.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9Y5B9
Reactivity Human, Mouse, Rat, Hamster, Monkey, Pig, Goat, Bovine, Dog
Host Goat
Clonality Polyclonal
Calculated MW 120kDa
Dilution ELISA (1:8000), IHC-P (3.75 µg/ml), WB (0.1-0.3 µg/ml)
Additional Information
Gene ID 11198
Other Names FACT complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, FACT 140 kDa subunit, FACTp140, Facilitates chromatin transcription complex subunit SPT16, hSPT16, SUPT16H, FACT140, FACTP140
Target/Specificity Human SUPT16H.
Reconstitution & Storage Store at -20°C. Minimize freezing and thawing.
PrecautionsSUPT16H / FACTP140 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SUPT16H
Synonyms FACT140, FACTP140
Function Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).
Cellular Location Nucleus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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Background

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene.

References

Orphanides G.,et al.Nature 400:284-288(1999).
Bienvenut W.V.,et al.Submitted (JUL-2007) to UniProtKB.
Bienvenut W.V.,et al.Submitted (MAR-2008) to UniProtKB.
Kang S.-W.,et al.Genes Cells 5:251-263(2000).
Orphanides G.,et al.Cell 92:105-116(1998).

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$ 395.00
Cat# ALS13428
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