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>   home   >   Products   >   Primary Antibodies   >   PIAS1 Antibody (C-Terminus)   

PIAS1 Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • IF - PIAS1 Antibody (C-Terminus) ALS13571
    Immunofluorescence of PIAS1 in rat kidney tissue with PIAS1 antibody at 20 ug/ml.
  • WB - PIAS1 Antibody (C-Terminus) ALS13571
    Western blot of PIAS1 in human kidney tissue lysate with PIAS1 antibody at 1 ug/ml in (A) the...
  • IHC - PIAS1 Antibody (C-Terminus) ALS13571
    Anti-PIAS1 antibody IHC of human colon.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession O75925
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 72kDa
Dilution IHC-P (5 µg/ml), WB (1-2 µg/ml),
Additional Information
Gene ID 8554
Other Names E3 SUMO-protein ligase PIAS1, 6.3.2.-, DEAD/H box-binding protein 1, Gu-binding protein, GBP, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein, PIAS1, DDXBP1
Target/Specificity Human PIAS1
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsPIAS1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIAS1
Synonyms DDXBP1
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity).
Cellular Location Nucleus speckle. Nucleus, PML body Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton
Tissue Location Expressed in numerous tissues with highest level in testis.
Citations (0)

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Background

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.

References

Liu B.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:10626-10631(1998).
Tan J.,et al.Mol. Endocrinol. 14:14-26(2000).
Wang P.,et al.BMC Genomics 10:518-518(2009).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:671-675(2006).

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$ 395.00
Cat# ALS13571
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