|Application ||WB, IHC-P|
|Dilution||IHC-P (10 µg/ml), WB (1:500-1:3000),|
|Other Names||RAD51-associated protein 1, RAD51-interacting protein, R51A1|
|Reconstitution & Storage||Aliquot and store at -20°C. Minimize freezing and thawing.|
|Precautions||RAD51AP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.|
|Cellular Location||Nucleus. Note=Colocalizes with RAD51 to multiple nuclear foci.|
|Tissue Location||Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes EMBL; AF006259; AAC39554.1; -; mRNA EMBL; AK096930; BAC04902.1; -; mRNA EMBL; AK291948; BAF84637.1; -; mRNA EMBL; CH471116; EAW88844.1; -; Genomic_DNA EMBL; BC006992; AAH06992.1; -; mRNA EMBL; BC016330; AAH16330.1; -; mRNA CCDS; CCDS44805.1; -. [Q96B01-1] CCDS; CCDS8529.1; -. [Q96B01-2] RefSeq; NP_001124334.1; NM_001130862.1. [Q96B01-1] RefSeq; NP_006470.1; NM_006479.4. [Q96B01-2] UniGene; Hs.730696; - ProteinModelPortal; Q96B01; - BioGrid; 115879; 15 DIP; DIP-35257N; - IntAct; Q96B01; 9 STRING; 9606.ENSP00000228843; - iPTMnet; Q96B01; - PhosphoSitePlus; Q96B01; - BioMuta; RAD51AP1; - DMDM; 68565925; - EPD; Q96B01; - MaxQB; Q96B01; - PaxDb; Q96B01; - PeptideAtlas; Q96B01; - PRIDE; Q96B01; - TopDownProteomics; Q96B01-1; -. [Q96B01-1] DNASU; 10635; - Ensembl; ENST00000228843; ENSP00000228843; ENSG00000111247. [Q96B01-1] Ensembl; ENST00000352618; ENSP00000309479; ENSG00000111247. [Q96B01-2] GeneID; 10635; - KEGG; hsa:10635; - UCSC; uc001qmu.4; human. [Q96B01-1] CTD; 10635; - DisGeNET; 10635; - GeneCards; RAD51AP1; - HGNC; HGNC:16956; RAD51AP1 HPA; HPA051499; - MIM; 603070; gene neXtProt; NX_Q96B01; - OpenTargets; ENSG00000111247; - PharmGKB; PA134871784; - eggNOG; ENOG410IICA; Eukaryota eggNOG; ENOG4111WAW; LUCA GeneTree; ENSGT00730000111239; - HOGENOM; HOG000116153; - HOVERGEN; HBG079699; - InParanoid; Q96B01; - KO; K20778; - OMA; EDFSMRK; - OrthoDB; EOG091G0OEX; - PhylomeDB; Q96B01; - TreeFam; TF335955; - BioCyc; ZFISH:ENSG00000111247-MONOMER; - Reactome; R-HSA-5685942; HDR through Homologous Recombination (HRR) Reactome; R-HSA-5693554; Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) Reactome; R-HSA-5693568; Resolution of D-loop Structures through Holliday Junction Intermediates Reactome; R-HSA-5693579; Homologous DNA Pairing and Strand Exchange ChiTaRS; RAD51AP1; human GenomeRNAi; 10635; - PRO; PR:Q96B01; - Proteomes; UP000005640; Chromosome 12 Bgee; ENSG00000111247; - CleanEx; HS_RAD51AP1; - ExpressionAtlas; Q96B01; baseline and differential Genevisible; Q96B01; HS GO; GO:0000790; C:nuclear chromatin; IDA:ParkinsonsUK-UCL GO; GO:0005654; C:nucleoplasm; TAS:Reactome GO; GO:0005634; C:nucleus; IC:UniProtKB GO; GO:0003690; F:double-stranded DNA binding; IDA:UniProtKB GO; GO:0003723; F:RNA binding; IDA:UniProtKB GO; GO:0003697; F:single-stranded DNA binding; IDA:UniProtKB GO; GO:0071479; P:cellular response to ionizing radiation; IDA:UniProtKB GO; GO:0006281; P:DNA repair; NAS:UniProtKB GO; GO:0000731; P:DNA synthesis involved in DNA repair; TAS:Reactome GO; GO:0000724; P:double-strand break repair via homologous recombination; NAS:UniProtKB GO; GO:0036297; P:interstrand cross-link repair; IMP:ParkinsonsUK-UCL GO; GO:0010569; P:regulation of double-strand break repair via homologous recombination; IDA:UniProtKB GO; GO:0000732; P:strand displacement; TAS:Reactome InterPro; IPR031419; RAD51_interact Pfam; PF15696; RAD51_interact; 1 1: Evidence at protein level; Alternative splicing; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding CHAIN 1 352 RAD51-associated protein 1 /FTId=PRO_0000097140 REGION 313 352 Interaction with RAD51 MOD_RES 19 19 Phosphoserine MOD_RES 21 21 Phosphoserine MOD_RES 66 66 Phosphothreonine MOD_RES 120 120 Phosphoserine MOD_RES 124 124 Phosphoserine MOD_RES 280 280 Phosphoserine MOD_RES 327 327 Phosphoserine VAR_SEQ 71 87 Missing (in isoform 2) /FTId=VSP_051739 VAR_SEQ 258 307 Missing (in isoform 3) /FTId=VSP_051740 VARIANT 68 68 K -> Q (in dbSNP:rs34810644) /FTId=VAR_056976 MUTAGEN 333 333 R->A: Strongly decreases interaction with RAD51; when associated with Q-336; A-345 and A-346. MUTAGEN 336 336 L->Q: Strongly decreases interaction with RAD51; when associated with A-333; A-345 and A-346. MUTAGEN 345 346 LH->AA: Strongly decreases interaction with RAD51; when associated with A-333; and Q-336. SEQUENCE 352 AA; 38457 MW; E582EE4BC459DD92 CRC64; MVRPVRHKKP VNYSQFDHSD SDDDFVSATV PLNKKSRTAP KELKQDKPKP NLNNLRKEEI PVQEKTPKKR LPEGTFSIPA SAVPCTKMAL DDKLYQRDLE VALALSVKEL PTVTTNVQNS QDKSIEKHGS SKIETMNKSP HISNCSVASD YLDLDKITVE DDVGGVQGKR KAASKAAAQQ RKILLEGSDG DSANDTEPDF APGEDSEDDS DFCESEDNDE DFSMRKSKVK EIKKKEVKVK SPVEKKEKKS KSKCNALVTS VDSAPAAVKS ESQSLPKKVS LSSDTTRKPL EIRSPSAESK KPKWVPPAAS GGSRSSSSPL VVVSVKSPNQ SLRLGLSRLA RVKPLHPNAT ST|
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Provided below are standard protocols that you may find useful for product applications.
May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.
Kovalenko O.V.,et al.Nucleic Acids Res. 25:4946-4953(1997).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Kovalenko O.V.,et al.Nucleic Acids Res. 34:5081-5092(2006).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
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