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RBBP8 / CTIP Antibody (Internal)

Rabbit Polyclonal Antibody

     
  • IF - RBBP8 / CTIP Antibody (Internal) ALS13940
    Immunofluorescence of RBBP8 in rat spleen tissue with RBBP8 antibody at 20 ug/ml.
  • WB - RBBP8 / CTIP Antibody (Internal) ALS13940
    Western blot analysis of RBBP8 in mouse spleen tissue lysate with RBBP8 antibody at (A) 1 and...
  • IHC - RBBP8 / CTIP Antibody (Internal) ALS13940
    Anti-RBBP8 / CTIP antibody IHC of human pancreas, duct.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q99708
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 102kDa
Dilution IHC-P (5 µg/ml), WB (1-2 µg/ml),
Additional Information
Gene ID 5932
Other Names DNA endonuclease RBBP8, 3.1.-.-, CtBP-interacting protein, CtIP, Retinoblastoma-binding protein 8, RBBP-8, Retinoblastoma-interacting protein and myosin-like, RIM, Sporulation in the absence of SPO11 protein 2 homolog, SAE2, RBBP8, CTIP
Target/Specificity Human RBBP8
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsRBBP8 / CTIP Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RBBP8
Synonyms CTIP
Function Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double- strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end- joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (PubMed:10764811, PubMed:10910365, PubMed:15485915, PubMed:16581787, PubMed:16818604, PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:20064462, PubMed:20829486). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity).
Cellular Location Nucleus Chromosome. Note=Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, Ref.29). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604)
Tissue Location Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level).
Volume 250 µl
Research Areas
Citations (0)

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Background

Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations.

References

Fusco C.,et al.Genomics 51:351-358(1998).
Schaeper U.,et al.J. Biol. Chem. 273:8549-8552(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Nusbaum C.,et al.Nature 437:551-555(2005).

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$ 395.00
Cat# ALS13940
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