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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   USP33 / VDU1 Antibody (clone 5B5)   

USP33 / VDU1 Antibody (clone 5B5)

Mouse Monoclonal Antibody

     
  • WB - USP33 / VDU1 Antibody (clone 5B5) ALS14085
    Western blot of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody,...
  • IF - USP33 / VDU1 Antibody (clone 5B5) ALS14085
    Immunofluorescence of monoclonal antibody to USP33 on HeLa cell. [antibody concentration 20 ug/ml]
  • IP - USP33 / VDU1 Antibody (clone 5B5) ALS14085
    Immunoprecipitation of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein...
  • IHC - USP33 / VDU1 Antibody (clone 5B5) ALS14085
    Anti-USP33 antibody IHC of human tonsil.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E, IP
Primary Accession Q8TEY7
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5B5
Calculated MW 107kDa
Dilution IF (20 µg/ml), IHC-P (5 µg/ml), WB (1:500-1:1000)
Additional Information
Gene ID 23032
Other Names Ubiquitin carboxyl-terminal hydrolase 33, 3.4.19.12, Deubiquitinating enzyme 33, Ubiquitin thioesterase 33, Ubiquitin-specific-processing protease 33, VHL-interacting deubiquitinating enzyme 1, hVDU1, USP33, KIAA1097, VDU1
Target/Specificity Human USP33
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsUSP33 / VDU1 Antibody (clone 5B5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP33
Synonyms KIAA1097, VDU1
Function Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta- arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Cellular Location Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Associates with centrosomes predominantly in S and G2 phases but less in G1 phase
Tissue Location Widely expressed.
Research Areas
Citations (0)

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Background

Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta- arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

References

Li Z.,et al.J. Biol. Chem. 277:4656-4662(2002).
Kikuno R.,et al.DNA Res. 6:197-205(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Curcio-Morelli C.,et al.J. Clin. Invest. 112:189-196(2003).

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$ 450.00
Cat# ALS14085
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