EMA / MUC1 Antibody (clone CM1)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, IF, IHC-F |
---|---|
Primary Accession | P15941 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | CM1 |
Calculated MW | 122kDa |
Dilution | IHC-Fr, IHC-P (10 µg/ml), |
Gene ID | 4582 |
---|---|
Other Names | Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227, Mucin-1 subunit alpha, MUC1-NT, MUC1-alpha, Mucin-1 subunit beta, MUC1-beta, MUC1-CT, MUC1, PUM |
Target/Specificity | VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids or as a result of chemical treatment of human milk MUC1. These MAbs do not recognize natural MUC1 protein isolated from human ... |
Reconstitution & Storage | +4°C, avoid freezing |
Precautions | EMA / MUC1 Antibody (clone CM1) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MUC1 |
---|---|
Synonyms | PUM |
Function | The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. |
Cellular Location | Apical cell membrane; Single-pass type I membrane protein. Note=Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells After endocytosis, internalized and recycled to the cell membrane Located to microvilli and to the tips of long filopodial protusions [Isoform Y]: Secreted. [Mucin-1 subunit beta]: Cell membrane. Cytoplasm. Nucleus. Note=On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus |
Tissue Location | Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
References
Lan M.S.,et al.J. Biol. Chem. 265:15294-15299(1990).
Ligtenberg M.J.L.,et al.J. Biol. Chem. 265:5573-5578(1990).
Gendler S.J.,et al.J. Biol. Chem. 265:15286-15293(1990).
Lancaster C.A.,et al.Biochem. Biophys. Res. Commun. 173:1019-1029(1990).
Wreschner D.H.,et al.Eur. J. Biochem. 189:463-473(1990).
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