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CFLAR / FLIP Antibody (clone 2F12)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | O15519 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 2F12 |
| Calculated MW | 55kDa |
| Dilution | IF (10 µg/ml), IHC-P (10 µg/ml), |
| Gene ID | 8837 |
|---|---|
| Other Names | CASP8 and FADD-like apoptosis regulator, Caspase homolog, CASH, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, CLARP, Cellular FLICE-like inhibitory protein, c-FLIP, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, CASP8 and FADD-like apoptosis regulator subunit p43, CASP8 and FADD-like apoptosis regulator subunit p12, CFLAR, CASH, CASP8AP1, CLARP, MRIT |
| Target/Specificity | Human CFLAR |
| Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Precautions | CFLAR / FLIP Antibody (clone 2F12) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CFLAR |
|---|---|
| Synonyms | CASH, CASP8AP1, CLARP, MRIT |
| Function | Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. |
| Tissue Location | Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle |
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Provided below are standard protocols that you may find useful for product applications.
Background
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
References
Shu H.-B.,et al.Immunity 6:751-763(1997).
Han D.K.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:11333-11338(1997).
Irmler M.,et al.Nature 388:190-195(1997).
Srinivasula S.M.,et al.J. Biol. Chem. 272:18542-18545(1997).
Hu S.,et al.J. Biol. Chem. 272:17255-17257(1997).
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