|Application ||WB, IHC-P, IF, E|
|Reactivity||Human, Mouse, Rat|
|Dilution||ELISA (1:10000), IF (1:100-1:500), IHC-P (10 µg/ml), WB (1:500-1:1000) ,|
|Other Names||Vinculin, Metavinculin, MV, VCL|
|Target/Specificity||Vinculin (Ab-821) Antibody detects endogenous levels of total Vinculin protein.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||VCL / Vinculin Antibody (aa786-835) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.|
|Cellular Location||Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Note=Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity) Colocalizes with LIMD1 in the focal adhesions.|
|Tissue Location||Metavinculin is muscle-specific.|
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Provided below are standard protocols that you may find useful for product applications.
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Weller P.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:5667-5671(1990).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Deloukas P.,et al.Nature 429:375-381(2004).
Moiseyeva E.P.,et al.J. Biol. Chem. 268:4318-4325(1993).
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
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