|Application ||IHC-P, IF, E|
|Dilution||ELISA (1:20000), IF (1:100-1:500), IHC-P (5 µg/ml)|
|Other Names||Cystatin-SN, Cystain-SA-I, Cystatin-1, Salivary cystatin-SA-1, CST1|
|Target/Specificity||CST1 Antibody detects endogenous levels of total CST1 protein.|
|Reconstitution & Storage||Store at -20°C for up to one year.|
|Precautions||CST1 / Cystatin SN Antibody (aa31-80) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.|
|Tissue Location||Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid|
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Provided below are standard protocols that you may find useful for product applications.
Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.
Saitoh E.,et al.Gene 61:329-338(1987).
Al-Hashimi I.,et al.J. Biol. Chem. 263:9381-9387(1988).
Deloukas P.,et al.Nature 414:865-871(2001).
Saitoh E.,et al.Biol. Chem. Hoppe-Seyler 369:191-197(1988).
Isemura S.,et al.J. Biochem. 110:648-654(1991).
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