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NDN / Necdin Antibody (clone 1B3)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q99608 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1B3 |
| Calculated MW | 36kDa |
| Dilution | IF (10 µg/ml), IHC-P (5 µg/ml), WB (1:500-1:1000) |
| Gene ID | 4692 |
|---|---|
| Other Names | Necdin, NDN |
| Target/Specificity | Human Necdin |
| Reconstitution & Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | NDN / Necdin Antibody (clone 1B3) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NDN |
|---|---|
| Function | Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Also functions as a transcription factor and directly binds to specific guanosine-rich DNA sequences (By similarity). |
| Cellular Location | Perikaryon. Nucleus. Note=Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix |
| Tissue Location | Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post-mitotic neurons |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences (By similarity).
References
Jay P.,et al.Nat. Genet. 17:357-360(1997).
Nakada Y.,et al.Gene 213:65-72(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Beneduzzi D.,et al.Eur. J. Endocrinol. 165:145-150(2011).
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