RICTOR Antibody (clone 1F3)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E, IP |
---|---|
Primary Accession | Q6R327 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 1F3 |
Calculated MW | 192kDa |
Dilution | IHC-P (5 µg/ml), WB (1:500-1:1000), |
Gene ID | 253260 |
---|---|
Other Names | Rapamycin-insensitive companion of mTOR, AVO3 homolog, hAVO3, RICTOR {ECO:0000312|EMBL:EAW55980.1} |
Target/Specificity | Human RICTOR |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
Precautions | RICTOR Antibody (clone 1F3) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RICTOR (HGNC:28611) |
---|---|
Function | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development. |
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Background
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
References
Sarbassov D.D.,et al.Curr. Biol. 14:1296-1302(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Schmutz J.,et al.Nature 431:268-274(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Ohara O.,et al.DNA Res. 9:47-57(2002).
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