|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat, Rabbit, Zebrafish, Hamster, Monkey, Chicken, Horse, Xenopus, Bovine|
|Dilution||ELISA (1:64000), IHC-P (5 µg/ml), WB (0.1-0.3 µg/ml)|
|Other Names||SUMO-conjugating enzyme UBC9, 6.3.2.-, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBE2I, UBC9, UBCE9|
|Target/Specificity||Human UBE2I / UBC9. This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||UBE2I / UBC9 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.|
|Cellular Location||Nucleus. Cytoplasm. Note=Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity).|
|Tissue Location||Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.|
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Provided below are standard protocols that you may find useful for product applications.
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.
Yasugi T.,et al.Nucleic Acids Res. 24:2005-2010(1996).
Tachibana M.,et al.Cytogenet. Cell Genet. 75:222-223(1996).
Watanabe T.K.,et al.Cytogenet. Cell Genet. 72:86-89(1996).
Masson M.,et al.Gene 190:287-296(1997).
Kovalenko O.V.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:2958-2963(1996).
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