NDUFA4 Antibody (aa32-81)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | O00483 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 9kDa |
Dilution | ELISA (1:10000), IHC-P (5 µg/ml), WB (1:500-1:1000) |
Gene ID | 4697 |
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Other Names | Cytochrome c oxidase subunit NDUFA4, Complex I-MLRQ, CI-MLRQ, NADH-ubiquinone oxidoreductase MLRQ subunit, NDUFA4 |
Target/Specificity | NDUFA4 Antibody detects endogenous levels of total NDUFA4 protein. |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
Precautions | NDUFA4 Antibody (aa32-81) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NDUFA4 |
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Function | Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol- cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix (PubMed:22902835). NDUFA4 is required for complex IV maintenance (PubMed:22902835). |
Cellular Location | Mitochondrion inner membrane; Single-pass membrane protein |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cytochrome c oxidase (COX, complex IV) is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. Required for complex IV maintenance.
References
Kim J.W.,et al.Biochem. Mol. Biol. Int. 43:669-675(1997).
Kanagarajah D.,et al.Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Scherer S.W.,et al.Science 300:767-772(2003).
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