|Application ||WB, IHC-P, IF|
|Reactivity||Human, Mouse, Rat|
|Dilution||IHC-P (5 µg/ml), WB (0.5-1 µg/ml),|
|Other Names||Vam6/Vps39-like protein, TRAP1-like protein, hVam6p, VPS39, KIAA0770, TLP, VAM6|
|Target/Specificity||Human VPS39. At least four isoforms of VPS39 are known to exist; this antibody will detect all four. VPS39 antibody is predicted not to cross-react with TRAP1.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||VPS39 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KIAA0770, TLP, VAM6|
|Function||Regulator of TGF-beta/activin signaling, inhibiting SMAD3- and activating SMAD2-dependent transcription. Acts by interfering with SMAD3/SMAD4 complex formation, this would lead to inhibition of SMAD3-dependent transcription and relieve SMAD3 inhibition of SMAD2-dependent promoters, thus increasing SMAD2- dependent transcription. Does not affect TGF-beta-induced SMAD2 or SMAD3 phosphorylation, nor SMAD2/SMAD4 complex formation.|
|Cellular Location||Cytoplasm. Lysosome membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein. Late endosome. Lysosome. Note=Colocalizes with TGFBR1 and TGFBR2 in cytoplasmic vesicular structures and most prominently in cortical vesicles|
|Tissue Location||Widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen.|
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May play a role in clustering and fusion of late endosomes and lysosomes. Regulator of TGF-beta/activin signaling, inhibiting SMAD3- and activating SMAD2-dependent transcription. Acts by interfering with SMAD3/SMAD4 complex formation, this would lead to inhibition of SMAD3-dependent transcription and relieve SMAD3 inhibition of SMAD2-dependent promoters, thus increasing SMAD2-dependent transcription. Does not affect TGF-beta-induced SMAD2 or SMAD3 phosphorylation, nor SMAD2/SMAD4 complex formation.
Caplan S.,et al.J. Cell Biol. 154:109-122(2001).
Dell'Angelica E.C.,et al.Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
Nagase T.,et al.DNA Res. 5:277-286(1998).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
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