|Application ||WB, IHC-P, IHC-Fr|
|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Chicken|
|Dilution||IHC-Fr (1:25-1:200), IHC-P (10 µg/ml), WB (1:100-1:1000)|
|Other Names||Desmin, DES|
|Target/Specificity||Reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma. Cross-reacts with most mammals.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||DES / Desmin Antibody (clone D9) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction.|
|Cellular Location||Cytoplasm, myofibril, sarcomere, Z line Cytoplasm. Cell membrane, sarcolemma. Note=Localizes in the intercalated disks which occur at the Z line of cardiomyocytes (PubMed:24200904, PubMed:26724190).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
Li Z.,et al.Gene 78:243-254(1989).
Li Z.,et al.J. Biol. Chem. 266:6562-6570(1991).
Vicart P.,et al.Hum. Genet. 98:422-429(1996).
Goldfarb L.G.,et al.Nat. Genet. 19:402-403(1998).
Li D.,et al.Circulation 100:461-464(1999).
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