|Application ||WB, IHC-P, IHC-Fr|
|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Chicken|
|Dilution||IHC-Fr (1:25-1:200), IHC-P (10 µg/ml), WB (1:100-1:1000)|
|Other Names||Desmin, DES|
|Target/Specificity||Reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma. Cross-reacts with most mammals.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||DES / Desmin Antibody (clone D9) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction.|
|Cellular Location||Cytoplasm, myofibril, sarcomere, Z line Cytoplasm. Cell membrane, sarcolemma. Note=Localizes in the intercalated disks which occur at the Z line of cardiomyocytes (PubMed:24200904, PubMed:26724190).|
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Provided below are standard protocols that you may find useful for product applications.
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
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Li Z.,et al.J. Biol. Chem. 266:6562-6570(1991).
Vicart P.,et al.Hum. Genet. 98:422-429(1996).
Goldfarb L.G.,et al.Nat. Genet. 19:402-403(1998).
Li D.,et al.Circulation 100:461-464(1999).
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