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PDIA3 / ERp57 Antibody (clone Map.ERP57)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IP |
|---|---|
| Primary Accession | P30101 |
| Reactivity | Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Bovine, Guinea Pig, Dog |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | Map.ERP57 |
| Calculated MW | 57kDa |
| Dilution | IHC-P (15 µg/ml), WB (1:20000), |
| Gene ID | 2923 |
|---|---|
| Other Names | Protein disulfide-isomerase A3, 5.3.4.1, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, PDIA3, ERP57, ERP60, GRP58 |
| Target/Specificity | Detects ~57 kD protein. |
| Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Precautions | PDIA3 / ERp57 Antibody (clone Map.ERP57) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PDIA3 (HGNC:4606) |
|---|---|
| Synonyms | ERP57, ERP60, GRP58 |
| Function | Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed:7487104, PubMed:11825568, PubMed:16193070, PubMed:27897272, PubMed:36104323). Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity (PubMed:35948544, PubMed:36104323). |
| Cellular Location | Endoplasmic reticulum. Endoplasmic reticulum lumen {ECO:0000250|UniProtKB:P11598}. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). |
| Tissue Location | Detected in the flagellum and head region of spermatozoa (at protein level) (PubMed:20400973). Expressed in liver, stomach and colon (at protein level). Expressed in gastric parietal cells and chief cells (at protein level) (PubMed:24188822) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
References
Hirano N.,et al.Biochem. Biophys. Res. Commun. 204:375-382(1994).
Bourdi M.,et al.Arch. Biochem. Biophys. 323:397-403(1995).
Koivunen P.,et al.Biochem. J. 316:599-605(1996).
Charnock-Jones D.S.,et al.Int. J. Biochem. Cell Biol. 28:81-89(1996).
Koivunen P.,et al.Genomics 42:397-404(1997).
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