|Application ||WB, IHC-P, IF, E|
|Reactivity||Human, Mouse, Rat|
|Dilution||ELISA (1:20000), IF (1:100-1:500), IHC-P (10 µg/ml), WB (1:500-1:1000) ,|
|Other Names||Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1/C2, HNRNPC, HNRPC|
|Target/Specificity||hnRNP C1/C2 Antibody detects endogenous levels of total hnRNP C1/C2 protein.|
|Reconstitution & Storage||Store at -20°C for up to one year.|
|Precautions||HNRNPC / HNRNP C Antibody (aa241-290) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6- methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non- coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre- mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'- UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).|
|Cellular Location||Nucleus. Note=Component of ribonucleosomes.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules.
Burd C.G.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:9788-9792(1989).
Swanson M.S.,et al.Mol. Cell. Biol. 7:1731-1739(1987).
Li W.B.,et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.