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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   NDFIP1 / N4WBP5 Antibody (N-Terminus)   

NDFIP1 / N4WBP5 Antibody (N-Terminus)

Rabbit Polyclonal Antibody

     
  • WB - NDFIP1 / N4WBP5 Antibody (N-Terminus) ALS15644
    Western blot analysis of NDFIP1 in PC-3 cell lysate with NDFIP1 antibody at (A) 0.5 and (B) 1 ug/ml.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q9BT67
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 25kDa
Dilution IHC-P (5 µg/ml), WB (0.5-1 µg/ml),
Additional Information
Gene ID 80762
Other Names NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M, NEDD4 WW domain-binding protein 5, Putative MAPK-activating protein PM13, Putative NF-kappa-B-activating protein 164, Putative NFKB and MAPK-activating protein, NDFIP1, N4WBP5
Target/Specificity Human NDFIP1. At least two isoforms of NDFIP1 are known to exist. NDFIP1 antibody is predicted to not cross-react with NDFIP2.
Reconstitution & Storage Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsNDFIP1 / N4WBP5 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDFIP1
Synonyms N4WBP5
Function Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity (PubMed:19706893). Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1- to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate (PubMed:20534535). Enhances the ubiquitination of BRAT1 mediated by E3 ubiquitin-protein ligases: NEDD4, NEDD4L and ITCH, and is required for the nuclear localization of ubiquitinated BRAT1 (PubMed:25631046).
Cellular Location Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q8R0W6}. Secreted. Note=Detected in exosomes and secreted via the exosomal pathway (PubMed:18819914)
Tissue Location Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta. Also expressed in fetal brain, kidney and lung.
Research Areas
Citations (0)

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Background

Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate.

References

Petroziello J.M.,et al.Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
Matsuda A.,et al.Oncogene 22:3307-3318(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Otsuki T.,et al.DNA Res. 12:117-126(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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$ 395.00
Cat# ALS15644
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