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PAN3 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, ICC |
|---|---|
| Primary Accession | Q58A45 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 96kDa |
| Dilution | IF (1:100-1:200), IHC-P (10 µg/ml), WB (1:500-1:3000) |
| Gene ID | 255967 |
|---|---|
| Other Names | PAB-dependent poly(A)-specific ribonuclease subunit PAN3 {ECO:0000255|HAMAP-Rule:MF_03181}, PAB1P-dependent poly(A)-nuclease {ECO:0000255|HAMAP-Rule:MF_03181}, PAN deadenylation complex subunit 3 {ECO:0000255|HAMAP-Rule:MF_03181}, hPan3, PAN3 {ECO:0000255|HAMAP-Rule:MF_03181} |
| Target/Specificity | Human PAN3. Predicted cross-reactivity based on amino acid sequence homology: mouse (98%), bovine (83%). |
| Reconstitution & Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Precautions | PAN3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PAN3 {ECO:0000255|HAMAP-Rule:MF_03181} |
|---|---|
| Function | Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenylation-dependent mRNA decapping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins. |
| Cellular Location | Cytoplasm, P-body {ECO:0000255|HAMAP- Rule:MF_03181, ECO:0000269|PubMed:14583602, ECO:0000269|PubMed:18625844} [Isoform 3]: Cytoplasm |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with PABP and to miRNA targets via its interaction with GW182 family proteins.
References
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Dunham A.,et al.Nature 428:522-528(2004).
Uchida N.,et al.J. Biol. Chem. 279:1383-1391(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Funakoshi Y.,et al.Genes Dev. 21:3135-3148(2007).
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