SCAF4 Antibody (aa601-650)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | O95104 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 126kDa |
Dilution | ELISA (1:40000), IHC-P (20 µg/ml), WB (1:500-1:1000) |
Gene ID | 57466 |
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Other Names | Splicing factor, arginine/serine-rich 15, CTD-binding SR-like protein RA4, SR-related and CTD-associated factor 4, SCAF4, KIAA1172, SFRS15 |
Target/Specificity | SFRS15 Antibody detects endogenous levels of total SFRS15 protein. |
Reconstitution & Storage | Store at -20°C for up to one year. |
Precautions | SCAF4 Antibody (aa601-650) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SCAF4 {ECO:0000303|PubMed:31104839, ECO:0000312|HGNC:HGNC:19304} |
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Function | Anti-terminator protein required to prevent early mRNA termination during transcription (PubMed:31104839). Together with SCAF8, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins (PubMed:31104839). Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation (PubMed:31104839). Independently of SCAF8, also acts as a suppressor of transcriptional readthrough (PubMed:31104839). |
Cellular Location | Nucleus. |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
May act to physically and functionally link transcription and pre-mRNA processing.
References
Tassone F.,et al.Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Hattori M.,et al.Nature 405:311-319(2000).
Hirosawa M.,et al.DNA Res. 6:329-336(1999).
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