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CCNT1 / Cyclin T1 Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • WB - CCNT1 / Cyclin T1 Antibody (C-Terminus) ALS16009
    Western blot analysis of CCNT1 in rat brain tissue lysate with CCNT1 antibody at 1 ug/ml in (A)...
  • IF - CCNT1 / Cyclin T1 Antibody (C-Terminus) ALS16009
    Immunofluorescence of CCNT1 in rat brain tissue with CCNT1 antibody at 20 ug/ml.
  • IHC - CCNT1 / Cyclin T1 Antibody (C-Terminus) ALS16009
    Anti-CCNT1 / Cyclin T1 antibody IHC staining of human testis.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession O60563
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 81kDa
Dilution IF (20 µg/ml), IHC-P (10 µg/ml), WB (1-2 µg/ml)
Additional Information
Gene ID 904
Other Names Cyclin-T1, CycT1, Cyclin-T, CCNT1
Target/Specificity Human CCNT1 / Cyclin T1
Reconstitution & Storage Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsCCNT1 / Cyclin T1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCNT1
Function Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).
Cellular Location Nucleus.
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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Background

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.

References

Wei P.,et al.Cell 92:451-462(1998).
Peng J.-M.,et al.Genes Dev. 12:755-762(1998).
Wu X.,et al.Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
Scherer S.E.,et al.Nature 440:346-351(2006).
Parada C.A.,et al.EMBO J. 18:3688-3701(1999).

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$ 395.00
Cat# ALS16009
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