|Application ||WB, IHC-P, IF, E|
|Reactivity||Human, Mouse, Rat|
|Dilution||IF (20 µg/ml), IHC-P (10 µg/ml), WB (1-2 µg/ml)|
|Other Names||Progesterone-induced-blocking factor 1, PIBF1, C13orf24, PIBF|
|Target/Specificity||Multiple isoforms of PIBF1 exists as a result of alternative splicing event.|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||PIBF1 / PIBF Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Isoform 1: Pericentriolar protein required to maintain mitotic spindle pole integrity (PubMed:21224392). Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation (PubMed:23110211). Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2 (PubMed:26297806).|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm. Secreted. Note=In progesterone-treated astrocytoma cells a 57 kDa protein and isoform 1 (90 kDa) have been described, both being located in the intracellular medium and secreted. Respective predominant forms are isoform 1 in the intracellular and the 57 kDa protein in the extracellular medium (PubMed:25218441). Isoform 4: Secreted Note=Secreted by progesterone-treated lymphocytes (PubMed:14634107).|
|Tissue Location||Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon (PubMed:11935316). Expressed in the first trimester pregnancy decidua (PubMed:12516630). Localized to extravillous cytotrophoblast (at protein level). Also found in syncytiotrophoblast and part of the villous cytotrophoblast Isoform 1 is expressed in first trimester and term villous trophoblast; trophoblast cells can additionally express other isoforms (PubMed:18817979). Overexpresed in solid tumors from stomach and uterus and in cells from ovary, cervical, breast, lymphoma and leukemia cancer (PubMed:25218441)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.
Rozenblum E.,et al.Hum. Genet. 110:111-121(2002).
Lachmann M.,et al.Int. J. Cancer 112:51-60(2004).
Szekeres-Bartho J.,et al.Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Dunham A.,et al.Nature 428:522-528(2004).
Szekeres-Bartho J.,et al.Int. Immunopharmacol. 1:1037-1048(2001).
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