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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PCMT1 Antibody (aa10-227)   

PCMT1 Antibody (aa10-227)

Rabbit Polyclonal Antibody

     
  • WB - PCMT1 Antibody (aa10-227) ALS16209
    Sample (30 ug of whole cell lysate). A:293T. 12% SDS PAGE. PCMT1 antibody diluted at 1:1000.
  • ICC - PCMT1 Antibody (aa10-227) ALS16209
    Immunofluorescence of methanol-fixed A431 using PCMT1 antibody at 1:200 dilution.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC
Primary Accession P22061
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 25kDa
Dilution ICC (1:100-1:1000), IHC-P (7.5 µg/ml), WB (1:500-1:3000)
Additional Information
Gene ID 5110
Other Names Protein-L-isoaspartate(D-aspartate) O-methyltransferase, PIMT, 2.1.1.77, L-isoaspartyl protein carboxyl methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase, PCMT1
Target/Specificity Human PCMT1
Reconstitution & Storage Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
PrecautionsPCMT1 Antibody (aa10-227) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCMT1
Function Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine- binding protein 1, stathmin, beta-synuclein and alpha-synuclein.
Cellular Location Cytoplasm.
Volume 50 µl
Research Areas
Citations (0)

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Background

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).

References

Ingrosso D.,et al.J. Biol. Chem. 264:20131-20139(1989).
Maclaren D.C.,et al.Biochem. Biophys. Res. Commun. 185:277-283(1992).
Takeda R.,et al.J. Biochem. 117:683-685(1995).
Shirasawa T.,et al.Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 395.00
Cat# ALS16209
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