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SF3B1 Antibody (aa75-124)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O75533 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 146kDa |
| Dilution | ELISA (1:10000), IHC-P (20 µg/ml), WB (1:500-1:1000) |
| Gene ID | 23451 |
|---|---|
| Other Names | Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, SF3b155, Spliceosome-associated protein 155, SAP 155, SF3B1, SAP155 |
| Target/Specificity | SAP 155 antibody detects endogenous levels of SAP 155. |
| Reconstitution & Storage | Store at -20°C. |
| Precautions | SF3B1 Antibody (aa75-124) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SF3B1 {ECO:0000303|PubMed:30567737, ECO:0000312|HGNC:HGNC:10768} |
|---|---|
| Function | Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:12234937, PubMed:27720643, PubMed:32494006, PubMed:34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:32494006, PubMed:34822310). Within the 17S U2 SnRNP complex, SF3B1 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed:12234937). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed:15146077, PubMed:33509932). Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity). |
| Cellular Location | Nucleus. Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
References
Wang C.,et al.Genes Dev. 12:1409-1414(1998).
Woessner J.,et al.Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 434:724-731(2005).
Yu W.,et al.Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
Das R.,et al.Mol. Cell 5:779-787(2000).
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