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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Nestin Antibody (clone 10C2)   

Nestin Antibody (clone 10C2)

Mouse Monoclonal Antibody

     
  • IHC - Nestin Antibody (clone 10C2) ALS16519
    Anti-Nestin antibody IHC of human small intestine.
  • ICC - Nestin Antibody (clone 10C2) ALS16519
    Immunostaining of intracellular nestin filaments in U251 cells
  • WB - Nestin Antibody (clone 10C2) ALS16519
    Immunoblotting of U251-cell lysate showing reactivity with nestin
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC, IHC-Fr
Primary Accession P48681
Other Accession 10763
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 10C2
Calculated MW 177439 Da
Dilution IHC-Fr (1:100 - 1:200), IHC-P (10 µg/ml), WB (1:1000 - 1:5000),
Additional Information
Gene ID 10763
Other Names NES, Nestin, Nbla00170
Target/Specificity Reacts exclusively with the human nestin protein. No cross-reactivity to rodent nestin.
Reconstitution & Storage PBS containing 0.09% sodium azide. Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
PrecautionsNestin Antibody (clone 10C2) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NES
Function Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
Tissue Location CNS stem cells.
Research Areas
Citations (0)

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Background

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

References

Dahlstrand J.,et al.J. Cell Sci. 103:589-597(1992).
Gregory S.G.,et al.Nature 441:315-321(2006).
Yaworsky P.J.,et al.Dev. Biol. 205:309-321(1999).
Ohira M.,et al.Cancer Lett. 197:63-68(2003).
Olsen J.V.,et al.Cell 127:635-648(2006).

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$ 395.00
Cat# ALS16519
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