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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CDKN1A / WAF1 / p21 Antibody (aa111-160)   

CDKN1A / WAF1 / p21 Antibody (aa111-160)

Rabbit Polyclonal Antibody

     
  • IHC - CDKN1A / WAF1 / p21 Antibody (aa111-160) ALS16537
    Anti-CDKN1A / WAF1 / p21 antibody IHC staining of human uterus, endometrium.
  • WB - CDKN1A / WAF1 / p21 Antibody (aa111-160) ALS16537
    Western blot of extracts from COLO/K562 cells, treated with EGF, using p21 Cip1 (Ab-145) Antibody.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P38936
Other Accession 1026
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 18119 Da
Dilution ELISA (1:20000), IHC-P (20 µg/ml), WB (1:500 - 1:1000),
Additional Information
Gene ID 1026
Other Names CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21
Target/Specificity CDKN1A Antibody detects endogenous levels of total CDKN1A protein.
Reconstitution & Storage PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year.
PrecautionsCDKN1A / WAF1 / p21 Antibody (aa111-160) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDKN1A
Synonyms CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WA
Function May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).
Cellular Location Cytoplasm. Nucleus.
Tissue Location Expressed in all adult tissues, with 5-fold lower levels observed in the brain
Volume 50 µl
Research Areas
Citations (0)

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Background

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

References

Harper J.W.,et al.Cell 75:805-816(1993).
El-Deiry W.S.,et al.Cell 75:817-825(1993).
Xiong Y.,et al.Nature 366:701-704(1993).
Jiang H.,et al.Mol. Cell. Differ. 1:285-299(1993).
Jiang H.,et al.Oncogene 10:1855-1864(1995).

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$ 395.00
Cat# ALS16537
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