|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||18119 Da|
|Dilution||ELISA (1:20000), IHC-P (20 µg/ml), WB (1:500 - 1:1000),|
|Other Names||CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21|
|Target/Specificity||CDKN1A Antibody detects endogenous levels of total CDKN1A protein.|
|Reconstitution & Storage||PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year.|
|Precautions||CDKN1A / WAF1 / p21 Antibody (aa111-160) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WA|
|Function||May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).|
|Cellular Location||Cytoplasm. Nucleus.|
|Tissue Location||Expressed in all adult tissues, with 5-fold lower levels observed in the brain|
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Provided below are standard protocols that you may find useful for product applications.
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
Harper J.W.,et al.Cell 75:805-816(1993).
El-Deiry W.S.,et al.Cell 75:817-825(1993).
Xiong Y.,et al.Nature 366:701-704(1993).
Jiang H.,et al.Mol. Cell. Differ. 1:285-299(1993).
Jiang H.,et al.Oncogene 10:1855-1864(1995).
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