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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RAD21 Antibody (aa521-570)   

RAD21 Antibody (aa521-570)

Rabbit Polyclonal Antibody

     
  • IHC - RAD21 Antibody (aa521-570) ALS16541
    Anti-RAD21 antibody IHC staining of human skin.
  • WB - RAD21 Antibody (aa521-570) ALS16541
    Western blot of extracts from Jurkat cells, using RAD21 Antibody.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O60216
Other Accession 5885
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 71690 Da
Dilution ELISA (1:1000), IHC-P (20 µg/ml), WB (1:500 - 1:1000),
Additional Information
Gene ID 5885
Other Names RAD21, CDLS4, HR21, KIAA0078, HHR21, NXP-1, NXP1, RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, RAD21 homolog (S. pombe), HRAD21, SCC1, SCC1 homolog
Target/Specificity RAD21 Antibody detects endogenous levels of total RAD21 protein.
Reconstitution & Storage PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year.
PrecautionsRAD21 Antibody (aa521-570) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD21
Synonyms HR21, KIAA0078, NXP1
Function Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
Cellular Location Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis
Volume 50 µl
Research Areas
Citations (0)

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Background

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

References

McKay M.J.,et al.Genomics 36:305-315(1996).
Sadano H.,et al.Biochem. Biophys. Res. Commun. 267:418-422(2000).
Nomura N.,et al.DNA Res. 1:223-229(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 395.00
Cat# ALS16541
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