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KSR2 Antibody (Ile694)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF |
|---|---|
| Primary Accession | Q6VAB6 |
| Other Accession | 283455 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 107632 Da |
| Dilution | IF (1:50 - 1:200), IHC-P (5 µg/ml), WB (1:500 - 1:1000), |
| Gene ID | 283455 |
|---|---|
| Other Names | KSR2, Kinase suppressor of ras 2, HKSR2 |
| Target/Specificity | Human KSR2 |
| Reconstitution & Storage | PBS, pH 7.2, 0.05% sodium azide. Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Precautions | KSR2 Antibody (Ile694) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KSR2 (HGNC:18610) |
|---|---|
| Function | Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 (PubMed:29433126). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF- kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production. |
| Cellular Location | Cytoplasm. Membrane; Peripheral membrane protein |
| Tissue Location | Mainly expressed in brain and kidney. |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8- mediated signaling. Acts as a negative regulator of MAP3K3- mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
References
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.E.,et al.Nature 440:346-351(2006).
Channavajhala P.L.,et al.J. Biol. Chem. 278:47089-47097(2003).
Channavajhala P.L.,et al.Biochem. Biophys. Res. Commun. 334:1214-1218(2005).
Brennan D.F.,et al.Nature 472:366-369(2011).
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