|Application ||WB, IHC-P, E|
|Calculated MW||133490 Da|
|Dilution||ELISA (1:40000), IHC-P (10 µg/ml), WB (1:500 - 1:1000),|
|Other Names||CEP135, Centrosomal protein of 135 kDa, CEP4, Centrosomal protein 4, Centrosome protein cep135, Centrosomal protein 135kDa, KIAA0635, MCPH8|
|Target/Specificity||CEP135 Antibody detects endogenous levels of total CEP135 protein.|
|Reconstitution & Storage||PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year.|
|Precautions||CEP135 Antibody (aa1081-1130) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Note=During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles|
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Provided below are standard protocols that you may find useful for product applications.
Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Ishikawa K.,et al.DNA Res. 5:169-176(1998).
Andersen J.S.,et al.Nature 426:570-574(2003).
Kleylein-Sohn J.,et al.Dev. Cell 13:190-202(2007).
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