|Application ||WB, IHC-P|
|Calculated MW||51111 Da|
|Dilution||IHC-P (10 µg/ml), WB (1:2000),|
|Other Names||CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 molecule|
|Reconstitution & Storage||PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide. Store at -20°C. Minimize freezing and thawing.|
|Precautions||CD4 Antibody (clone 5D9) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum|
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Provided below are standard protocols that you may find useful for product applications.
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Maddon P.J.,et al.Cell 42:93-104(1985).
Littman D.R.,et al.Cell 55:541-541(1988).
Ansari-Lari M.A.,et al.Genome Res. 6:314-326(1996).
Ansari-Lari M.A.,et al.Genome Res. 7:268-280(1997).
Hodge T.W.,et al.Hum. Immunol. 30:99-104(1991).
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