|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||17298 Da|
|Dilution||ELISA (1:1000), IHC-P (10 µg/ml), WB (1:500 - 1:1000),|
|Other Names||Nucleoside diphosphate kinase B, NDK B, NDP kinase B, 220.127.116.11, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, 18.104.22.168, nm23-H2, NME2, NM23B|
|Target/Specificity||NM23 Antibody detects endogenous levels of total NM23 protein.|
|Reconstitution & Storage||PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year.|
|Precautions||NME2 Antibody (aa91-140) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.|
|Cellular Location||Cytoplasm. Nucleus. Cell projection, lamellipodium. Cell projection, ruffle. Note=Isoform 3 is mainly cytoplasmic and isoform 1 and isoform 3 are excluded from the nucleolus. Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates.|
|Tissue Location||Isoform 1 and isoform 3 are ubiquitously expressed.|
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