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NOS2 / iNOS Antibody (clone 4E5)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E, FC |
|---|---|
| Primary Accession | P35228 |
| Other Accession | 4843 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone Names | 4E5 |
| Calculated MW | 131117 Da |
| Dilution | ELISA (1:10000), Flo (1:200 - 1:400), IHC-P (10 µg/ml), WB (1:500 - 1:2000), |
| Gene ID | 4843 |
|---|---|
| Other Names | NOS2, Hepatocyte NOS, HEP-NOS, Inducible NOS, NOS, type II, NOS type II, Inducible NO synthase, NOS2A, INOS, NOS |
| Target/Specificity | Human NOS2 / iNOS |
| Reconstitution & Storage | PBS, 0.05% sodium azide, 0.05% protein stabilizer. Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. |
| Precautions | NOS2 / iNOS Antibody (clone 4E5) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NOS2 (HGNC:7873) |
|---|---|
| Synonyms | NOS2A |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004, PubMed:7682706, PubMed:7504305). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109). |
| Cellular Location | Cytoplasm, cytosol. Note=Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. |
| Tissue Location | Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets Expressed in chondrocytes (PubMed:7504305) |
Provided below are standard protocols that you may find useful for product applications.
Background
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
References
Sherman P.A.,et al.Biochemistry 32:11600-11605(1993).
Geller D.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:3491-3495(1993).
Charles I.G.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:11419-11423(1993).
Maier R.,et al.Biochim. Biophys. Acta 1208:145-150(1994).
Park C.S.,et al.Biochem. Biophys. Res. Commun. 205:85-91(1994).
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