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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RNF20 Antibody (N-Terminus)   

RNF20 Antibody (N-Terminus)

Rabbit Polyclonal Antibody

     
  • IHC - RNF20 Antibody (N-Terminus) ALS16761
    Anti-RNF20 antibody IHC staining of human skin.
  • IF - RNF20 Antibody (N-Terminus) ALS16761
    Immunofluorescence of RNF20 in human liver tissue with RNF20 antibody at 20 ug/ml.
  • WB - RNF20 Antibody (N-Terminus) ALS16761
    Western blot analysis of RNF20 in human liver tissue lysate with RNF20 antibody at (A) 1 and (B)...
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q5VTR2
Other Accession 56254
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 113662 Da
Dilution IF (20 µg/ml), IHC-P (10 µg/ml), WB (1 - 2 µg/ml),
Additional Information
Gene ID 56254
Other Names RNF20, BRE1, BRE1-A, HBRE1, KAIA2779, RING finger protein 20, BRE1A, Homolog of S. cerevisiae BRE1
Target/Specificity Multiple isoforms of RNF20 are known to exist.
Reconstitution & Storage PBS, 0.02% sodium azide. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsRNF20 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF20
Synonyms BRE1A
Function Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Cellular Location Nucleus
Tissue Location Expressed in the normal brain and also in malignant gliomas (at protein level)
Research Areas
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Background

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

References

Wu H.,et al.Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Zhu B.,et al.Mol. Cell 20:601-611(2005).

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$ 395.00
Cat# ALS16761
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