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NOS2 / iNOS Antibody (clone K13-A)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, IHC-F |
|---|---|
| Primary Accession | P35228 |
| Other Accession | 4843 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone Names | K13-A |
| Calculated MW | 131117 Da |
| Dilution | IHC-P (1:200), |
| Gene ID | 4843 |
|---|---|
| Other Names | NOS2, Hepatocyte NOS, HEP-NOS, Inducible NOS, NOS, type II, NOS type II, Inducible NO synthase, NOS2A, INOS, NOS |
| Reconstitution & Storage | 20 mM Tris-HCl, pH 8.0, 20 mg/ml BSA stabilzer, 0.05% NaN3 preservative. +4°C, avoid freezing |
| Precautions | NOS2 / iNOS Antibody (clone K13-A) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NOS2 (HGNC:7873) |
|---|---|
| Synonyms | NOS2A |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004, PubMed:7682706, PubMed:7504305). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109). |
| Cellular Location | Cytoplasm, cytosol. Note=Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. |
| Tissue Location | Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets Expressed in chondrocytes (PubMed:7504305) |
| Volume | 50 µl |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
References
Sherman P.A.,et al.Biochemistry 32:11600-11605(1993).
Geller D.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:3491-3495(1993).
Charles I.G.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:11419-11423(1993).
Maier R.,et al.Biochim. Biophys. Acta 1208:145-150(1994).
Park C.S.,et al.Biochem. Biophys. Res. Commun. 205:85-91(1994).
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