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FSP27 / CIDEC Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, ICC |
|---|---|
| Primary Accession | Q96AQ7 |
| Other Accession | 63924 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 26754 Da |
| Dilution | ICC (1:100 - 1:1000), IF (1:100 - 1:1000), IHC-P (7.5 µg/ml), WB (1:500 - 1:3000), |
| Gene ID | 63924 |
|---|---|
| Other Names | CIDEC, CIDE3, CIDE-3, FSP27, Cell death activator CIDE-3, CIDEX, Fat specific protein 27 |
| Target/Specificity | Human CIDEC / FSP27 |
| Reconstitution & Storage | PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal. Aliquot and freeze at -20° C. Avoid freeze-thaw cycles. |
| Precautions | FSP27 / CIDEC Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CIDEC {ECO:0000303|PubMed:20049731, ECO:0000312|HGNC:HGNC:24229} |
|---|---|
| Function | Lipid transferase specifically expressed in white adipose tissue, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion (PubMed:18334488, PubMed:19843876, PubMed:20049731, PubMed:23399566, PubMed:30361435). Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage (PubMed:18334488, PubMed:19843876, PubMed:20049731, PubMed:23399566). Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by undergoing liquid-liquid phase separation (LLPS) and promoting directional net neutral lipid transfer from the smaller to larger lipid droplets (PubMed:18334488, PubMed:19843876, PubMed:20049731, PubMed:23399566). The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair (PubMed:18334488, PubMed:19843876, PubMed:20049731, PubMed:23399566). Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1 (PubMed:23399566). May also act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH (By similarity). When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment (PubMed:12429024). |
| Cellular Location | Lipid droplet. Endoplasmic reticulum {ECO:0000250|UniProtKB:P56198}. Nucleus {ECO:0000250|UniProtKB:P56198} Note=Diffuses quickly on lipid droplet surface, but becomes trapped and clustered at lipid droplet contact sites, thereby enabling its rapid enrichment at lipid droplet contact sites {ECO:0000250|UniProtKB:P56198} |
| Tissue Location | Expressed mainly in adipose tissue, small intestine, heart, colon and stomach and, at lower levels, in brain, kidney and liver. |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1. May act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment. As mature adipocytes, that express high CIDEC levels, are quite resistant to apoptotic stimuli, the physiological significance of its role in apoptosis is unclear. May play a role in the modulation of the response to osmotic stress by preventing NFAT5 to translocate into the nucleus and activate its target genes expression.
References
Liang L.,et al.Biochem. J. 370:195-203(2003).
Liang L.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Keller P.,et al.J. Biol. Chem. 283:14355-14365(2008).
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