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IKBKG / NEMO / IKK Gamma Antibody (aa51-100)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q9Y6K9 |
| Other Accession | 8517 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 48198 Da |
| Dilution | ELISA (1:1000), IF (1:100 - 1:500), IHC-P (5 µg/ml), WB (1:500 - 1:1000), |
| Gene ID | 8517 |
|---|---|
| Other Names | IKBKG, AMCBX1, FIP3, Fip3p, IkappaB kinase gamma, IKKgamma, IKK-gamma, NEMO, NF-kappa-B essential modifier, I-kappa-B kinase gamma-subunit, I-kappa-B kinase subunit gamma, IkB kinase gamma subunit, IKKAP1, Incontinentia pigmenti, IP2, IPD2, NF-kappa- ... |
| Target/Specificity | Detects endogenous levels of total IKK-gamma protein. |
| Reconstitution & Storage | PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year. |
| Precautions | IKBKG / NEMO / IKK Gamma Antibody (aa51-100) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | IKBKG (HGNC:5961) |
|---|---|
| Synonyms | FIP3, NEMO |
| Function | Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor (PubMed:9751060, PubMed:14695475, PubMed:20724660, PubMed:21518757). Its binding to scaffolding polyubiquitin plays a key role in IKK activation by multiple signaling receptor pathways (PubMed:16547522, PubMed:18287044, PubMed:19033441, PubMed:21606507, PubMed:27777308, PubMed:19185524, PubMed:33567255). Can recognize and bind both 'Lys-63'-linked and linear polyubiquitin upon cell stimulation, with a much higher affinity for linear polyubiquitin (PubMed:16547522, PubMed:18287044, PubMed:27777308, PubMed:19033441, PubMed:21606507, PubMed:19185524). Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3 (PubMed:19854139). Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys- 27'-linked polyubiquitination (PubMed:20724660). |
| Cellular Location | Cytoplasm. Nucleus Note=Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress. |
| Tissue Location | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'- linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B- mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.
References
Li Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:1042-1047(1999).
Jin D.-Y.,et al.J. Biomed. Sci. 6:115-120(1999).
Rothwarf D.M.,et al.Nature 395:297-300(1998).
Smahi A.,et al.Nature 405:466-472(2000).
Ye Z.,et al.Biochem. Biophys. Res. Commun. 275:223-227(2000).
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