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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   c-CBL Antibody (clone 3B12)   

c-CBL Antibody (clone 3B12)

Mouse Monoclonal Antibody

     
  • IHC - c-CBL Antibody (clone 3B12) ALS17066
    Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
  • IHC - c-CBL Antibody (clone 3B12) ALS17066
    Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
  • IF - c-CBL Antibody (clone 3B12) ALS17066
    Immunofluorescence of HeLa cells using C-CBL mouse monoclonal antibody (green).
  • WB - c-CBL Antibody (clone 3B12) ALS17066
    Western blot using C-CBL mouse monoclonal antibody against RAJI (1), RAW264.7 (2), K562 (3),...
  • WB - c-CBL Antibody (clone 3B12) ALS17066
    Western blot using C-CBL monoclonal antibody against human C-CBL (AA: 684-865) recombinant protein.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC, E, Flo
Primary Accession P22681
Other Accession 867
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 3B12
Calculated MW 99633 Da
Dilution ELISA (1:10000), Flo (1:200 - 1:400), ICC (1:200 - 1:1000), IF (1:1000), IHC-P (1:50), WB (1:500 - 1:2000),
Additional Information
Gene ID 867
Other Names CBL, C-CBL, Oncogene CBL2, RING finger protein 55, Proto-oncogene c-Cbl, RNF55, CBL2, FRA11B, NSLL
Target/Specificity Human CBL
Reconstitution & Storage Ascites, 0.03% sodium azide. Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
Precautionsc-CBL Antibody (clone 3B12) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBL
Synonyms CBL2, RNF55
Function Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
Cellular Location Cytoplasm. Cell membrane. Note=Colocalizes with FGFR2 in lipid rafts at the cell membrane
Volume 250 µl
Research Areas
Citations (0)

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Background

Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.

References

Blake T.J.,et al.Oncogene 6:653-657(1991).
Taylor T.D.,et al.Nature 440:497-500(2006).
Howlett C.J.,et al.Biochem. Biophys. Res. Commun. 257:129-138(1999).
Joazeiro C.A.,et al.Science 286:309-312(1999).
Donovan J.A.,et al.J. Biol. Chem. 269:22921-22924(1994).

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$ 395.00
Cat# ALS17066
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