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PCK1 Antibody

Rabbit Polyclonal Antibody

     
  • IHC-P - PCK1 Antibody ALS17156
    Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
    detail
  • IHC-P - PCK1 Antibody ALS17156
    Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P35558
Other Accession 5105
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 69195 Da
Dilution IHC-P (10 µg/ml),
Additional Information
Gene ID 5105
Other Names PCK1, PEPCKC, Phosphopyruvate carboxylase, PEP carboxykinase, PEPCK-C, PEPCK1
Target/Specificity Human PCK1
Reconstitution & Storage PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsPCK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCK1 {ECO:0000303|PubMed:8490617, ECO:0000312|HGNC:HGNC:8724}
Function Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis (PubMed:30193097, PubMed:24863970, PubMed:26971250, PubMed:28216384). Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle (PubMed:30193097, PubMed:24863970, PubMed:26971250, PubMed:28216384). At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed:30193097). At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate (PubMed:30193097). Acts as a regulator of formation and maintenance of memory CD8(+) T-cells: up- regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis (By similarity). The resultant phosphoenolpyruvate flows to glycogen and pentose phosphate pathway, which is essential for memory CD8(+) T-cells homeostasis (By similarity). In addition to the phosphoenolpyruvate carboxykinase activity, also acts as a protein kinase when phosphorylated at Ser-90: phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as donor (PubMed:32322062). The protein kinase activity regulates lipogenesis: upon phosphorylation at Ser-90, translocates to the endoplasmic reticulum and catalyzes phosphorylation of INSIG proteins (INSIG1 and INSIG2), thereby disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis- related genes (PubMed:32322062).
Cellular Location Cytoplasm, cytosol. Endoplasmic reticulum Note=Phosphorylation at Ser-90 promotes translocation to the endoplasmic reticulum.
Tissue Location Major sites of expression are liver, kidney and adipocytes.
Research Areas
Citations (0)
citation

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Background

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

References

Stoffel M.,et al.Hum. Mol. Genet. 2:1-4(1993).
Ting C.-N.,et al.Genomics 16:698-706(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 414:865-871(2001).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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Discontinued
Cat# ALS17156
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